Effects of stress on thymic cell populations. A – Flow cytometry scatter plots for thymic cells from one control (top) and one stressed (bottom) mice. The plots show 5% of the total number of events, for one representative mouse from each group. Gates R1 (cell debris) and R2 (whole cells) were positioned by standard procedures. B – Histograms showing the number of annexin V+ (top) and PI+(bottom) cells in the corresponding R2 regions. C – Effects of stress on the percentage of events in regions R1 and R2. D – Effects of stress on the percentage of annexin V+ and PI+ cells in region R2. The experiment was done 3 times using 3 ≤ n ≤ 5 mice per group. Data for one representative experiment of the 3 are shown.