Skip to main content

Table 1 IL-1β-induced TNF secretion is decreased in lipid-loaded macrophages

From: Decreased inducibility of TNF expression in lipid-loaded macrophages

  TNF concentration (pg/mL)
Treatment Experiment 1 Experiment 2 Experiment 3
LPDS 5.9 5.4 6.4
LPDS + IL-1β 14.2 165.3 23.0
Ac-LDL 5.5 5.6 5.4
Ac-LDL + IL-1β 8.4 54.5 14.6
Inhibition 41% 67% 36%
  1. Macrophages were incubated for 48 h in the presence of 5% LPDS ± 50 μg/mL ac-LDL. They were then rinsed with serum-free medium, and incubated for 24 h in serum-free medium with or without IL-1β (10 ng/mL). Culture medium was collected, centrifuged at 4000 g for 10 min, and aliquots of the supernatant were frozen for analysis of secreted TNF by ELISA.