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Figure 1 | BMC Immunology

Figure 1

From: Increased production of pro-inflammatory cytokines and enhanced T cell responses after activation of human dendritic cells with IL-1 and CD40 ligand

Figure 1

Comparison of different activation signals on gene expression in DC. Normalized gene expression levels from 2–7 independent experiments with averages indicated by a horizontal bar. DC were cultured with LPS+IFN-γ (n = 2); CD40L+IFN-γ (n = 3); CD40L+IL-1β (n = 7); CD40L (n = 7); IL-1β (n = 4); medium n = 4 for 8 hours and total RNA was extracted and hybridized to biotin-labeled RNA probes. After RNase treatment, digested probes were separated on a 5% acrylamide/8 M urea gel, blotted to a positively charged membrane for detection with CDP-Star substrate solution, and exposed to film or directly to the Kodak Image Station. Controls include yeast RNA incubated with probes followed or not by RNase treatment. Relative gene expression levels were calculated by dividing the net intensity of a gene (calculated with Kodak 1D software) with the net intensity of L32 in the same lane.

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BMC Immunology

ISSN: 1471-2172

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