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Figure 6 | BMC Immunology

Figure 6

From: Increased production of pro-inflammatory cytokines and enhanced T cell responses after activation of human dendritic cells with IL-1 and CD40 ligand

Figure 6

CD40L+IL-1β stimulated DC are highly effective APCs for CD8+ T cell priming. HLA-A2+ cord blood-derived immature DC were treated with CD40L+IL-1β, CD40L or medium for 16 hours, pulsed with Flu-MP peptide, washed and used to prime naïve autologous T cells. On day 7, T cell cultures were analyzed for the presence of antigen-specific CD8+ T cells using Flu-MP-HLA-A2 tetramers. Representative dot plots are shown of CD8 vs. Flu-MP tetramer (A), and CD45RA vs. Flu-MP tetramer (gated on CD8+ cells) (B). All were gated on live, PI- cells; quadrants were set based on background staining with control mAbs. Percent positivity is indicated in corresponding quadrants.

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BMC Immunology

ISSN: 1471-2172

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