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Table 1 Effects of DC activation on priming of CD8+ T cell responses

From: Increased production of pro-inflammatory cytokines and enhanced T cell responses after activation of human dendritic cells with IL-1 and CD40 ligand

Exp. (a)

DC used in priming (b)

 

Percent of CD8+ cells binding Flu-MP-HLA-A2 tetramer

Absolute number of Flu-MP specific cells

Fold expansion Flu-MP specific T cells

1

CD40L + IL-1β

 

2.39

5020

Not determined

 

CD40L

 

1.68

2900

"

 

Immature

 

0.84

1190

"

2

CD40L + IL-1β

 

0.28

1400

7.0

 

CD40L

 

0.24

528

2.6

 

Immature

 

0.25

656

3.3

3

CD40L + IL-1β

 

0.26

594

2.9

 

CD40L

 

0.14

309

1.5

 

Immature

 

0.00

0

0

4

CD40L + IL-1β

IgG

1.39

2870

19.1

 

CD40L

"

0.64

945

6.3

 

Immature

"

0.61

1130

7.5

 

CD40L + IL-1β

Anti-IL-12

0.57

1270

8.5

 

CD40L

"

0.78

1200

8.0

 

Immature

"

0.41

700

4.7

  1. (a) Neonatal umbilical cord blood was used for exp. 1–3, adult mobilized peripheral blood was used in exp. 4. (b) Monocyte-derived DC cultured in GM-CSF+IL-4 were used as immature DC (unstimulated), or after activation with CD40L (1 ug/ml) or CD40L + IL-1β (1 ug/ml, 10 ng/ml respectively) for 24 hours. In experiment 4, polyclonal neutralizing Abs to IL-12 or normal goat IgG (1 μg/ml), were added at time of priming.