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Table 1 Effects of DC activation on priming of CD8+ T cell responses

From: Increased production of pro-inflammatory cytokines and enhanced T cell responses after activation of human dendritic cells with IL-1 and CD40 ligand

Exp. (a) DC used in priming (b)   Percent of CD8+ cells binding Flu-MP-HLA-A2 tetramer Absolute number of Flu-MP specific cells Fold expansion Flu-MP specific T cells
1 CD40L + IL-1β   2.39 5020 Not determined
  CD40L   1.68 2900 "
  Immature   0.84 1190 "
2 CD40L + IL-1β   0.28 1400 7.0
  CD40L   0.24 528 2.6
  Immature   0.25 656 3.3
3 CD40L + IL-1β   0.26 594 2.9
  CD40L   0.14 309 1.5
  Immature   0.00 0 0
4 CD40L + IL-1β IgG 1.39 2870 19.1
  CD40L " 0.64 945 6.3
  Immature " 0.61 1130 7.5
  CD40L + IL-1β Anti-IL-12 0.57 1270 8.5
  CD40L " 0.78 1200 8.0
  Immature " 0.41 700 4.7
  1. (a) Neonatal umbilical cord blood was used for exp. 1–3, adult mobilized peripheral blood was used in exp. 4. (b) Monocyte-derived DC cultured in GM-CSF+IL-4 were used as immature DC (unstimulated), or after activation with CD40L (1 ug/ml) or CD40L + IL-1β (1 ug/ml, 10 ng/ml respectively) for 24 hours. In experiment 4, polyclonal neutralizing Abs to IL-12 or normal goat IgG (1 μg/ml), were added at time of priming.