Figure 4
From: Flt3+ macrophage precursors commit sequentially to osteoclasts, dendritic cells and microglia
Osteoclast differentiation of day 6-FL cells (A) Day 6-FL cells cultivated for 6 days in the presence of M-CSF and RANKL formed mixed population of large multinucleated cells together with mononucleated cells. Note that 100% of the cells are TRAP-positive, as indicated by their blue staining. (B) When grown on top of dentin slices, cells actively resorbed dentin as shown by arrows pointing to the toluidin blue stained resorption pits.