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Figure 6 | BMC Immunology

Figure 6

From: Flt3+ macrophage precursors commit sequentially to osteoclasts, dendritic cells and microglia

Figure 6

Characterization of day 11-FL cells differentiated into microglial cells (A) CD11b staining of day 11-FL cells cultured for 3 days in presence of fresh media alone. (B) CD11b staining of day 11-FL cells cultured for 3 days in presence of 50% GCCM. (C) Actin-phalloidin staining of day-11 FL cells cultured for 3 days in presence of 50% GCCM. Note the presence of actin-filled spines and pseupodia (white arrow). (D) Electron microscopy photomicrograph of day 11-FL cells cultured for 3 days in presence of 50% GCCM. Differentiated cells exhibited cell processes bearing spines of 0.1 μm diameter (black arrows). (E) After 6 days in the presence of GCCM, cells exhibited longer processes expanding from the cell bodies as shown by phase-contrast microscopy. Insert shows typical thread-like structures of 0.05 μm diameter (dashed arrow). (F) FACS profiles compare immunophenotype of day 11-FL cells and adherent cells obtained after cultivating day 11-FL cells for 3 days in the presence of 50% GCCM.

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