Figure 1
BCR engagement induces growth arrest associated with apoptosis in BCL 1 .3B3 (A) BCL1.3B3 cells were plated at 1 × 105 cells/ml for 24 hours and then incubated with 15 μg/ml of rabbit anti-IgM (anti-IgM – closed circle) or control rabbit anti-ovalbumin (anti-ova – open circle) antibody on day 0. Samples were harvested, stained with Trypan blue and counted on the indicated days. (B) BCL1.3B3 cells were plated at 2 × 105 cells/ml and incubated with 25 μg of rabbit anti-IgM antibody or control rabbit anti-ova antibody for 24 hours. Samples were then harvested, stained with 7-AAD and Hoechst 33342, and analyzed by flow cytometry. In the dot plot analysis of 7-AAD (x-axis) and Hoechst (y-axis) fluorescence, the apoptotic population is circled.