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Figure 4 | BMC Immunology

Figure 4

From: Activation of the Syk tyrosine kinase is insufficient for downstream signal transduction in B lymphocytes

Figure 4

Syk isolated from unstimulated (K395A/3F)Syk-infected clones can phosphorylate an exogenous substrate Syk was immunoprecipitated from an unstimulated (-) or stimulated (+, 15 μg anti-IgM for 0.5 minutes) vector control clone and from a clone expressing the (K395A/3F)Syk molecule (clone 2.1.2). The ICKA was performed with (lanes 3, 4, 7, and 8) and without (lanes 1, 2, 5, and 6) the human erythrocyte band-GST substrate, c3B. The arrowhead and arrow mark the migration position of phosphorylated Syk and phosphorylated c3B, respectively.

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BMC Immunology

ISSN: 1471-2172

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