Figure 4
From: Enhanced immunogenicity of a functional enzyme by T cell epitope modification
Proteolytic activity and stability of the variant GP002 as compared to the parent enzyme. The variant enzyme, GP002, was compared to BPN' Y217L for both proteolytic activity and thermostability. Figure 4A shows proteolytic activity as determined by a suc-AAFP-pNA hydrolysis assay. Figure 4B shows the results of the thermostability test performed at close to physiological conditions. Residual activity was determined over time, and the half-life was determined by linear regression.