Figure 5

The introduction of a T cell epitope into the BPN' Y217L backbone results in a complex T cell epitope map. Two groups of five guinea pigs were immunized with 5 ug of either the parent or variant enzyme every two weeks for a total of three immunizations. At eight weeks, spleens were removed and splenocytes were tested in vitro for proliferative responses to BPN' Y217L peptides. The average proliferation is recorded above for each peptide across the five animals. Note that an epitope was created for the BPN' Y217L peptide number 25, where the single amino acid change was effected. The proliferative response was also boosted across the entire range of the enzyme.