Figure 8

Priming of IgG responses to the parent enzyme, BPN' Y217L, by the T cell epitope modified variant GP002. Four sets of four Guinea pigs were immunized with 5 ug of enzyme on various schedules. All groups were immunized three times, once every two weeks. Group A was immunized all three times with BPN' Y217L. Group B was immunized first with GP002, and then twice with BPN' Y217L. Group C was immunized the first two times with GP002, and the third time with BPN' Y217L. Finally, Group D was immunized all three times with GP002. After testing the sera for whole IgG by ELISA on BPN' Y217L coated plates, titers were calculated