Skip to main content
Figure 3 | BMC Immunology

Figure 3

From: Auto-protective redox buffering systems in stimulated macrophages

Figure 3

Transcriptional modulation of important redox proteins in RAW 264.7 either undifferentiated, or differentiated cells. RAW 264.7 cells were cultured (2.5 × 106 cells/well) in medium, or medium supplemented with either LPS (5 μg/ml – hached boxes), or IFN-γ (50 U/ml – green boxes) or a mixture of IFN-γ (50 U/ml) + LPS (5 μg/ml) (black boxes). After 24 h, the cells were recovered and washed. Total cellular RNA were extracted and used for semi-quantitative RT-PCR evaluation of the redox proteins indicated. Vertical axis indicate the ratio of the redox protein mRNA in cells cultured in medium with the three stimulating conditions, to the same redox protein mRNA in cells cultured in medium alone. HPRT mRNA was used as internal standard.

Back to article page