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Table 2 Differentiation of RAW 264 7 cells toward secreting-activated macrophages following culture with LPS, IFN-γ or LPS+IFN-γ

From: Auto-protective redox buffering systems in stimulated macrophages

      nitric oxide metabolism
  TNF production (pg/ml) nitrites/nitrates (μM) citruline (μM)
Sample type 0 h 6 h 24 h 48 h 0 h 6 h 24 h 48 h 0 h 6 h 24 h 48 h
medium 156+/ 12 215+/ 18 332+/ 168 462 +/ 250 ND ND ND ND 20+/ 4 22+/ 5 25+/ 7 33+/ 8
LPS 156+/ 12 1480+/ 187 1646+/ 266 1733+/ 284 ND ND 26+/ 5 38+/ 18 20+/ 4 35+/ 12 47+/ 11 86+/ 31
IFN 156+/ 12 402+/ 28 681 +/ 42 819+/ 126 ND ND 4+/ 2 12+/ 3 20+/ 4 25+/ 5 34+/ 9 42+/ 8
LPS/IFN 156+/ 12 1392+/ 224 1502+/ 335 1754+/ 201 ND ND 13+/ 9 33+/ 15 20+/ 4 37+/ 12 43+/ 7 72+/ 22
  1. ND: not detectable RAW 264.7 cells (250 000 cells/well) were cultured with medium or medium supplemented with either IFN-γ (50 U/ml), or LPS (5 μg/ml) or a mixture of IFN-γ (50 U/ml) + LPS (5 μg/ml). Cell-free culture supernatants were harvested at 0, 6, 24, and 48 hours. The concentrations of TNF-α (measured using an ELISA kit), nitrites/nitrates (measured using the Griess reagent) and citrulline (measured using colorimetric reaction), were evaluated in each culture supernatant as described in Methods. Data represent means +/- SEM of five independent experiments.