Auto-protective redox buffering systems in stimulated macrophages

BMC Immunology

Table 2 Differentiation of RAW 264 7 cells toward secreting-activated macrophages following culture with LPS, IFN-γ or LPS+IFN-γ

ND: not detectable RAW 264.7 cells (250 000 cells/well) were cultured with medium or medium supplemented with either IFN-γ (50 U/ml), or LPS (5 μg/ml) or a mixture of IFN-γ (50 U/ml) + LPS (5 μg/ml). Cell-free culture supernatants were harvested at 0, 6, 24, and 48 hours. The concentrations of TNF-α (measured using an ELISA kit), nitrites/nitrates (measured using the Griess reagent) and citrulline (measured using colorimetric reaction), were evaluated in each culture supernatant as described in Methods. Data represent means +/- SEM of five independent experiments.

ISSN: 1471-2172