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Table 3 Activities of the redox enzymes catalase, Gpx and SOD, in differentiated NO resistant RAW 264 7 cells

From: Auto-protective redox buffering systems in stimulated macrophages

  catalase (U/mg) Gpx (U/mg) SOD (U/mg)
time (hours) 6 24 48 6 24 48 6 24 48
control 873+/-100 761+/-128 767+/-96 257+/-116 379+/-95 300+/-32 249+/-111 263+/-47 326+/-76
LPS 5 1563+/-408 1036+/-670 738+/-90 335+/-206 249+/-55 323+/-52 454+/-174 205+/-136 231+/-169
IFN 50 598+/81 555+/ 92 612+/76 420+/ 30 218+/46 248+/17 1637+/512 288+/ 85 66+/37
LPS 5 /IFN 50 778+/67 698+/167 849+/ 33 280+/13 213+/61 268+/ 18 1833+/1601 284+/ 190 236+/115
  1. RAW 264.7 cells were cultured (10 × 106 cells in 50 ml) with medium or medium supplemented with either IFN-γ (50 U/ml), or LPS (5 μg/ml) or a mixture of IFN-γ (50 U/ml) + LPS (5 μg/ml). Cells were harvested at 6, 24, and 48 hours. Activities of the enzymes : catalase (decomposition of H2O2), Gpx (oxidation of NADPH) and SOD (reaction with chromophore) were determined on cell lysates as described in Methods. Results are expressed as means +/- SEM of five independent experiments.