Auto-protective redox buffering systems in stimulated macrophages

BMC Immunology

Table 3 Activities of the redox enzymes catalase, Gpx and SOD, in differentiated NO resistant RAW 264 7 cells

RAW 264.7 cells were cultured (10 × 10⁶ cells in 50 ml) with medium or medium supplemented with either IFN-γ (50 U/ml), or LPS (5 μg/ml) or a mixture of IFN-γ (50 U/ml) + LPS (5 μg/ml). Cells were harvested at 6, 24, and 48 hours. Activities of the enzymes : catalase (decomposition of H₂O₂), Gpx (oxidation of NADPH) and SOD (reaction with chromophore) were determined on cell lysates as described in Methods. Results are expressed as means +/- SEM of five independent experiments.

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