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Figure 3 | BMC Immunology

Figure 3

From: Efficient adenovirus-mediated gene transfer into primary T cells and thymocytes in a new coxsackie/adenovirus receptor transgenic model

Figure 3

Transduction with Ad5.UbP.GFP and Ad5.CMV5.GFP of Th1 and Th2 lines derived from line 18 mice. Th1 and Th2 lines were derived from line 18 DO11.hCARΔcyt double transgenic mice by antigenic stimulation in polarizing conditions for 3 weeks. One week after the last antigenic stimulation, the resting Th1 and Th2 lines were transduced for 30' with Ad5.UbP.GFP or Ad5.CMV5.GFP at a MOI of 4 and incubated for 24 h at 37°C/5%CO2. Half of the cells transduced with Ad5.CMV5.GFP were stimulated for 4 h with PMA/ionomycin before analysis. The histograms represent the GFP expression of the Th1 (left panels) and Th2 (right panels) lines transduced with the indicated adenovirus with or without stimulation with PMA/ionomycin. The percentage of positive cells and mean fluorescence intensity are indicated in Table 1.

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BMC Immunology

ISSN: 1471-2172

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