Efficient adenovirus-mediated gene transfer into primary T cells and thymocytes in a new coxsackie/adenovirus receptor transgenic model

Table 1 Transduction of Th1 and Th2 lines with Ad5.CMV5.GFP and Ad5.UbP.GFP

^*Th1 and Th2 lines derived from line 18 DO11.10/hCARΔcyt transgenic mice were transduced and GFP expression was analyzed as described in Fig. 4. The lines were activated by incubation on anti-CD3 coated plates (10 μg/ml), addition of irradiated BALB/c splenocytes and 5 μg/ml of OVA peptide or 50 ng/ml PMA and 5 μM ionomycin for 24 h at 37°C/5% CO₂. ^aPercentage of GFP positive cells (and mean fluorescence intensity of the GFP positive population) are indicated. ^bND – not determined.

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