Efficient adenovirus-mediated gene transfer into primary T cells and thymocytes in a new coxsackie/adenovirus receptor transgenic model

Table 2 Anatomic Distribution of Ad5.UbP.GFP-transduced DO11.hCARΔcyt versus Control DO11.10 T Cell Populations following Adoptive Transfer

		% of Total KJ1-26⁺ Cells in Each Lymphoid Tissue Microenvironment^a
Tissue/Microenvironent		DO11.10	GFP-positive DO11.hCARΔcyt
Spleen
	PALS	92^b	91
	Primary Nodules/Follicles	3	5
	Marginal Zone	4	2
	Red Pulp	1	3
Inguinal LN
	Diffuse Cortex/Paracortex	98	97
	Primary Nodules/Follicles	2	3
	Medullary Cords	0	0
Mesenteric LN
	Diffuse Cortex/Paracortex	97	96
	Primary Nodules/Follicles	3	4
	Medullary Cords	0	0
Peyer's Patch
	Paranodular zones (T cell)	99	98
	Nodules (B cell)	1	2

^a Two days following transfer of 5 × 10⁶ DO11.10 or Ad5.UbP.GFP-transduced DO11.hCARΔcyt CD4⁺ T cells into BALB/c recipients, animals were sacrificed and the recovered lymphoid tissues were processed for microscopic analysis as described in Material and Methods. ^b Numbers are the fraction of the total cell population in each tissue. Definition of the lymphoid tissue microenvironments follows the nomenclature of Weiss. Data are the mean from 3–4 animals per group (standard deviations <16%).

ISSN: 1471-2172