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Figure 2 | BMC Immunology

Figure 2

From: IL-4 dependent alternatively-activated macrophages have a distinctive in vivo gene expression phenotype

Figure 2

Mouse array analysis and real time PCR verfication. (A) Chart of the genes that showed the highest differential expression between NeMφ and thioglycollate-recruited macrophages. Values represent the difference between the gene expression in WT NeMφ and thioglycollate-recruited Mφ (arbitrary units). (B) Chart showing the difference between the gene expression in WT NeMφ and IL-4 -/- NeMφ. Genes selected for real-time PCR analysis are marked with an asterisk (*). (C) An example of a section of the Atlas array that contains MIP-1α (c), MIP-1β (d), MIP-2 (e). The left panel is probed with WT NeMφ and the right panel with IL-4-/- NeMφ. This section also contains the genes: prothymosin beta 4 (a), insulin-like growth factor IA (b), GADPH (f), myosin I alpha (g), and ornithine decarboxylase (h), which are all not differentially expressed. (D) Verification of Atlas array results by real-time PCR. cDNA samples from F4/80 purified macrophages from either resident peritoneal cells (con) or from parasite implanted mice (NeMφ) were diluted and normalized to contain equal levels of β-actin transcript (data not shown) before quantification of the different genes. Expression levels are shown in arbitrary units that are based on a comparison with β-actin expression (designated as 10,000 units). The results shown represent the mean values of 2 experiments with independent sources of experimental macrophage RNA.

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