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Figure 3 | BMC Immunology

Figure 3

From: Human CD81 directly enhances Th1 and Th2 cell activation, but preferentially induces proliferation of Th2 cells upon long-term stimulation

Figure 3

CD81 costimulation occurs via direct action on T cells. PBMC were treated with magnetic beads for depletion of non-T cells, then T cells or non-T cells were treated for one hour on ice with 10 μg/ml 5A6 anti-CD81 mAb, washed twice, and re-mixed. The cultures were then stimulated for 6 h in the presence of 0.1 μg/ml SEB, and CD69 and IL-2 expression were quantitated by intracellular staining. Note that pre-treatment of T cells, but not non-T cells, resulted in augmentation of CD69 expression and IL-2 production. Error bars represent S.D. Results are representative of three experiments.

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BMC Immunology

ISSN: 1471-2172

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