Figure 4

Effects of blocking mAbs against IFN receptors on up-regulation of mRNA expression by CpG ODN. PBMC were stimulated with IFN-α2 (200 IU/ml), IFN-γ (100 IU/ml) or CpG ODN 2006 (2 μg/ml) in the presence or absence of blocking mAbs against IFN-α/β receptor or IFN-γ receptor for 6 hours. The mRNA levels of IFIT1 (A), IP-10 (B) and OAS1 (C) were determined by SYBR^® green-based real-time quantitative RT-PCR. The results are shown as the mean ± SEM of three independent experiments and expressed as -fold increase above the mRNA level in the control cells. In this experiment, up-regulation of IFIT1 and IP-10 by CpG ODN 2006 was not blocked by the addition of the isotype-matched control mAbs (data not shown). * p < 0.05 vs CpG ODN 2006 stimulated. NS: not significant.