Figure 1

Representative examples of tube- and plate-based CFC results. (A) Whole blood from a CMV seropositive donor was stimulated (or not) with CMV pp65 peptide mix in 15 ml conical polypropylene tubes (top panels) or a deep-well 96-well polypropylene plate (bottom panels). (B) PBMC from another CMV seropositive donor were stimulated as above in 15 ml conical polypropylene tubes (top panels) or a 96-well round-bottom tissue culture plate (bottom panels). (C) Whole blood from an HIV-seropositve donor was stimulated (or not) with HIV p55 gag peptide mix in 15 ml conical polypropylene tubes (top panels) or a deep-well 24-well round-bottom polypropylene plate (bottom panels). Backgrounds and response to peptide mix were essentially equivalent in tubes and plates in each case. All data are gated on CD3⁺CD8⁺ cells.