Figure 3
From: NF-κB p50 facilitates neutrophil accumulation during LPS-induced pulmonary inflammation
Effect of p50 deficiency on ICAM-1 expression in the lungs. cDNA was prepared from RNA in lungs collected from WT and p50-deficient mice. ICAM-1 expression was measured by semi-quantitative multiplex RT-PCR. The autoradiograph (A) shows representative samples, with each lane containing amplicons of ICAM-1 message and 18S rRNA from cDNA reverse transcribed from lung RNA of an independent mouse. The graph (B) depicts ICAM-1 message for each sample expressed relative to the densitometric value of 18S rRNA from the same PCR reaction tube. Bars depict mean and SEM from 4–7 mice per group, and asterisk (*) denotes significant difference from WT mice.