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Figure 5 | BMC Immunology

Figure 5

From: NF-κB p50 facilitates neutrophil accumulation during LPS-induced pulmonary inflammation

Figure 5

Effect of p50 deficiency on chemokine responsiveness of blood neutrophils. (A) Effect of p50 deficiency on concentrations of neutrophil chemokines in the blood. E. coli LPS was instilled intratracheally to WT and p50-deficient mice, blood was collected after 6 hours, and the concentrations of MIP-2 and KC were quantified by ELISA. Asterisks (*) denote significant differences from WT mice. (B) Effect of p50 deficiency on the ability of blood neutrophils to respond to the chemokine KC. Since chemoattractants induce cell stiffening which results in neutropenia, circulating neutrophils (PMN) were counted before and after the intravenous injection of recombinant murine KC. Circulating neutrophils were quantified by differential counting of blood samples collected from the inferior vena cava. There were no significant effects of p50 deficiency. (C) Effect of p50 deficiency on the ability of blood neutrophils to respond to the chemokine KC during LPS-induced pulmonary inflammation. E. coli LPS was instilled intratracheally to WT and p50-deficient mice, and 6 hours later neutrophil responsiveness to recombinant murine KC was assessed as described above. Asterisks (*) denote significant differences from WT mice.

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