Figure 2From: Fluorescent derivatization of a protease antigen to track antigen uptake and processing in human cell linesLC/MS/MS analysis of tryptic digested FITC-Subtilisin. Top panel: Full base peak MS spectrum and base peak search spectrum for FITC molecule at m/z = 390.0 Da. Middle panel: A FITC-subtilisin peptide (137–145, 1221.2 Da) was identified at RT = 23.88 min. Bottom panel: Subsequent MS/MS fragmentation on the same peptide molecule confirmed its sequence.Back to article page