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Table 6 Subtilisin Peptides Identified from Subtilisin Antigen Uptake in Human Cells

From: Fluorescent derivatization of a protease antigen to track antigen uptake and processing in human cell lines

Cells

HLA

Peptide Source

Subtilisin peptide

Peptide sequence

Theory (Da)

Found (Da)

Ion match

8.1.6

DR3

Cell extract

114–126

AIANNMDVINMSL

1405.7

1406.1

10/24

Preiss

DR4

Cell extract

108–122

INGIEWAIANNMDVI

1672.8

1671.6

9/28

8.1.6

DR3

Cell extract

43–53

KVAGGASMVPS

1003.5

1004.3

10/20

KG-1

DR11, DR14

DR-bound

70–84 *

GTVAALNNSIGVLGV

1384.6

1384.78

12/28

  1. * Identified by Q-TOF analysis. The others were identified by LC-MS analysis.