Figure 5
From: Age-associated alterations in CXCL1 chemokine expression by murine B cells
Similar proliferation and differentiation between young and aged splenic B cells cultured in EL-4. FACS-sorted distinct B cell subsets (1000/well) from three young and aged mice were cultured in EL-4 culture system. Proliferation was measured by [3H] thymidine uptake after 3 days culture. Data represent the mean and variations (SD) from triplicate cultures. The data presented are representative of two independent experiments (A). Purified splenic B cells (4 × 104/well) from three young and aged mice were cultured with irradiated EL-4 thymoma cells in the presence of LPS and macrophage supernatant for 3 and 5 days. The cultured cells were stained with anti-IgG1 and anti IgM Abs. Percentages of IgG1+ IgM+ B cells are indicated (B). Absolute numbers of IgG1+ B cells in culture. Total numbers of IgG1+ B cells per well were calculated from a mixture of 20 wells in each group (C). Culture supernatants were measured by ELISA for IgG production. Data are representative of two independent experiments (D).