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Table 1 Flow cytometric analysis of transfected Jurkat T cell lines

From: Identification of genes differentially expressed in T cells following stimulation with the chemokines CXCL12 and CXCL10

% Positive (MFI)

Cell Line

CD3

CXCR4

CXCR3

CXCR2

Jurkat-Neo

98 (138)

99 (118)

2 (5)

4 (4)

Jurkat-CXCR3

98 (154)

99 (124)

99 (24)

5 (8)

Jurkat-Bcl2

96 (144)

96 (124)

5 (6)

7 (5)

  1. CXCR3-, Bcl2-and Neo-transfected Jurkat T cells were stained using IgG FITC-labeled antibodies to CD3, CXCR4, CXCR3, and CXCR2 and analyzed for cell surface protein expression via flow cytometric analysis. The data are expressed as % positive (mean fluorescence intensity). It should be noted that the CXCR3-transfected Jurkat cells expressed low levels of CXCR3 on their cells surface suggesting a lower receptor density in comparison to CXCR4.