% Positive (MFI) |
---|
Cell Line | CD3 | CXCR4 | CXCR3 | CXCR2 |
---|
Jurkat-Neo | 98 (138) | 99 (118) | 2 (5) | 4 (4) |
Jurkat-CXCR3 | 98 (154) | 99 (124) | 99 (24) | 5 (8) |
Jurkat-Bcl2 | 96 (144) | 96 (124) | 5 (6) | 7 (5) |
- CXCR3-, Bcl2-and Neo-transfected Jurkat T cells were stained using IgG FITC-labeled antibodies to CD3, CXCR4, CXCR3, and CXCR2 and analyzed for cell surface protein expression via flow cytometric analysis. The data are expressed as % positive (mean fluorescence intensity). It should be noted that the CXCR3-transfected Jurkat cells expressed low levels of CXCR3 on their cells surface suggesting a lower receptor density in comparison to CXCR4.