Figure 2

FACS-sorting of naïve and memory B-cells from splenocytes. A single B-cell suspension prepared from a fresh spleen was isolated using the Human B-cell Isolation Kit (See methods) and subjected to 3-color cell sorting. Memory B-cells from the splenic B-cells were gated on the IgM^highIgD^lowCD27⁺ fraction, while naïve B-cells were gated at IgM^lowIgD^highCD27^-. FACS-sorted populations were over 90 % pure, judging from post sort immunophenotyping.