Figure 4

Confirmation of the Microarray analysis by semi quantitative RT-PCR. aRNA amplified from GC, MNZ and MGZ -cells was reversely transcribed and amplified by PCR. The human HPRT gene was used as the comparative standard. Five fold serially dilutions (4 dilutions) of each cDNA amplified with gene specific primers and analyzed by electrophoresis in 2% agarose gel. The transcripts had either an almost exclusive expression in one compartment (ARK2 in GC, CCL20 in MNZ and CMRF-35H in MGZ), or they were expressed in all compartments with a significant differential expression in one – for example, SET and FAIM in GC, Cyclin G2 in MNZ and NM23-H1, CARD11 and GAS2 in MGZ.