Figure 9
Effect of Bay 11 and SB203580 on the enhancing effect of epinephrine (Epi) on production of IL-6 (A), IL-8 (B), and IL-13 (C) from IL-1β-induced HMC-1 cells. To each well of a 6 well culture plate, two ml of HMC-1 mast cells (0.75 × 106 cells/ml) were cultured alone (Medium), or in the presence of IL-1β (10 ng/ml), Epi (1 × 10-5 M), Bay 11 (1 × 10-5 M), SB 203580 (1 × 10-5 M), and the combinations of these reagents for 24 hrs in triplicate. Supernatants were harvested for measuring IL-6, IL-8, and IL-13 by ELISA. IL-8 production was not detected in the Medium, Epi, Bay 11 alone groups, while IL-13 production was not detected in the Bay 11 and SB 203580 alone groups. In A, by Student's t-test analysis, * indicates p < 0.005, when compared with the IL-1β-treated group, and + and ++ indicate p < 0.0005 and <0.00005, when compared with the IL-1β plus Epi group. In B, * indicates p < 0.05, when compared with both the IL-1β-treated group, and the IL-1β plus Epi group. In C, * indicates p < 0.005, when compared with the IL-1β-treated group, and + and ++ indicate p < 0.00005 and <0.0001, when compared with the IL-1β plus Epi group.