Figure 6

Antigen-specific antibody augments BM-APC-induced T cell proliferation. T cell proliferation responses from deer mice 223 and 224 were assessed as described in Figure 5 using BM-APC. KLH-specific antiserum or normal deer mouse serum were diluted 1:2,000 in DMM-5 and incubated with dilutions of KLH for 1 hour in 96-well plates at room temperature. BM-APC and T cells were added to the wells and incubated 72 hours, and proliferation was assessed by MTS assay.