Figure 7

Schematic overview of the process for culturing autologous deer mouse BM-APC and T cells. Ten days post immunization, lymph nodes, spleens and bone marrow are harvested from euthanized deer mice. The lymph node cells are cultured with antigen for four days, while the splenocytes and bone marrow cells are frozen at -70°C. On day 4, the blasting lymph node T cells are recovered and cultured with fresh antigen and thawed splenocytes (without mitomycin-C treatment). Simultaneously, bone marrow cells are thawed and cultured with GM-CSF. Expansion of the T cells is performed with huIL-2 and the BM-APC with GM-CSF for 14 days. These cells are then used for proliferation experiments.