Figure 3
Expression levels of cytokines and related molecules in control rats. A) Cycle threshold (CT) values of pro-inflammatory cytokines and SOCS3 mRNA in various structures and organs in Lewis rats. Quantifications were carried out from 30 ng of RNA (phenol extraction). Each value is the mean of seven values. Data are means ± SEM. B) CT values of pro-inflammatory cytokine and receptor mRNAs in hypothalamus from Sprague-Dawley rats. Quantification was carried out from 30 ng of RNA (RNeasy extraction; QIAGEN). Each value is the mean of seven values. Data are means ± SEM.