Figure 5

Quantification of cytokine-related molecule mRNAs in various pathophysiological conditions. Studies were in accordance with French ethical guidelines and approved by a Medical Committee of the French Army Medical Research Centre (CRSSA). Sham-animals were handled in the same conditions as injured animals without injury exposure. Conditions for 40% total body surface thermal injury (TI) and 8 Gy whole-body irradiation (WBI) were described previously [12, 22]. A 30 minutes contention was achieved in the irradiation device. Data are means ± SEM, n = 6. Statistical significance was assessed using ANOVA or Student's t-test (* p < 0.05; ** p < 0.01; *** p < 0.001). A.) 1; increase in spleen TNFa mRNA 3 h after 8 Gy WBI, 2; increase in liver SOCS3 mRNA 24 h after 40% total body surface TI, 3; ileum increase in IL1RA mRNA 23 h after 8 Gy WBI. B.) 1; increase in hypothalamus IL1a mRNA 3 h after a 30 minutes contention, 2; decrease in liver IL6 mRNA 6 h after 40% total body surface TI, 3; increase in hypothalamus IL6-receptor mRNA 2 h after 8 Gy WBI.