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Table 2 Primer sequences used for house-keeping genes and cytokine related molecules real-time PCR assays

From: Rat pro-inflammatory cytokine and cytokine related mRNA quantification by real-time polymerase chain reaction using SYBR green

Gene name

5'-3' primer sequence

Position cDNA-genea

Accession Numberb

βactin

FW

AAGTCCCTCACCCTCCCAAAAG

3474–3570

J00691

 

RW

AAGCAATGCTGTCACCTTCCC

Exon

V01217

CypA

FW

TATCTGCACTGCCAAGACTGAGTG

381–507

M19533

 

RW

CTTCTTGCTGGTCTTGCCATTCC

Int. span.

NW_047430

GAPDH

FW

GTATTGGGCGCCTGGTCACC*

73–274

AB017801

 

RW

CGCTCCTGGAAGATGGTGATGG

Exon

NW_047696.1

HPRT

FW

CTCATGGACTGATTATGGACAGGAC

211–333

S79292

 

RW

GCAGGTCAGCAAAGAACTTATAGCC

Int. span.

NW_047696.1

SOCS3

FW

CCTCCAGCATCTTTGTCGGAAGAC

581–679

AF075383

 

RW

TACTGGTCCAGGAACTCCCGAATG

Exon

AJ249240

C/EBPβ

FW

TGGACAAGCTGAGCGACGAG

656–760

NM_024125

 

RW

TGTGCTGCGTCTCCAGGTTG

Exon

NW_047660.1

cFos

FW

TTCACCCTGCCTCTTCTCAATGAC

849–931

X06769

 

RW

GCCTTCAGCTCCATGTTGCTAATG

Int. span.

NW_047762

cJun

FW

CAATGGGCACATCACCACTACAC

604–725

X17163

 

RW

TCTGGCTATGCAGTTCAGCTAGG

Exon

NW_047717.1

COX-2

FW

TGTATGCTACCATCTGGCTTCGG

981–1074

S67722

 

RW

GTTTGGAACAGTCGCTCGTCATC

Exon

NW_047397.1

I-KB

FW

TGAGTACCTGGACTTGCAGAACG

334–409

AF246634

 

RW

TGTAGATGCCTCTCCAAGGATGG

Int. span.

NW_047557.1

NOS-2*

FW

CATTGGAAGTGAAGCGTTTCG

2103–2197

L12562

 

RW

CAGCTGGGCTGTACAAACCTT

Int. span.

AABR03074184

  1. Note. FW forward primer; RW reverse primer; CypA, cyclophilin A; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HPRT, hypoxanthine-guanine phosphorybosyl transferase; SOCS3, Suppressor of cytokine signalling 3; C/EBPβ (NF-IL6 rodent homologue); COX-2, cyclooxygenase 2; I-KB, KappaB inhibitors; NOS-2, nitric oxide synthase 2. a Position of amplification product within cDNA sequence (upper line) and within genomic sequences (lower line). Exon; both primers bound on the same exon, Int. span.: primers bound on different exons, , b Genbank accession number of cDNA (upper line) and genomic sequences or contigs (lower line), available at http://www.ncbi.nlm.nih.gov/ *primers are stemmed from mouse sequence. ‡ gene without intron.