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Figure 1 | BMC Immunology

Figure 1

From: Endothelial cells present antigens in vivo

Figure 1

Genetic immunization with a β-gal expression vector induces strong humoral responses in wild type mice (FVB) and transgenic mice (VWF-lacZ and TIE2-lacZ) that express β-gal in their endothelial cells A. Equivalent β-gal specific antisera are generated in FVB and VWF-lacZ mice. ELISAs were performed on antisera from mice immunized 6 weeks earlier with a gene gun. Closed markers represent sera from mice immunized with the expression vector CMV-β gal. Open markers represent preimmune sera (VWF-lacZ) or sera from mice immunized with a control expression vector (CMV-GFP, FVB). Similar results were obtained in two independent experiments. B. The humoral response matures in wild type and transgenic mice. Titers of antibodies in control sera (0) and sera from mice immunized once (1') or twice (2') with the CMV-β-gal expression vector DNA are shown. Similar results were obtained in two independent experiments. C. TIE2-lacZ mice respond against β-gal. Antisera of wild type (FVB) and TIE2-lacZ mice immunized once 6 weeks previously are shown. Closed symbols represent sera assayed on plates coated with β-gal, open symbols represent assays on control, BSA-coated plates. Similar results were obtained in two independent experiments. Titers of sera from pre- or un-immunized mice were <10-2 (not shown). D. Soluble β-gal competes for antibodies in sera from FVB and transgenic mice equivalently. Sera from immunized and boosted mice were mixed with soluble β-gal at the concentrations indicated and incubated overnight at 4°C. Free antibodies were then detected by adding the mixture to β-gal coated wells.

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