Figure 2
From: Diversity and repertoire of IgW and IgM VH families in the newborn nurse shark
Alignment of VH and partial CH1 of IgM cDNA clones. The deduced amino acid sequences of 25 IgM cDNA clones (Genbank accession # AY609247-AY609274) were aligned with the conventional adult nurse shark IgM (Genbank accession #M92851 [32]) in ClustalW. Dashes (-) indicate identity with the first sequence and gaps introduced to correct alignment are indicated by slashes (/). Identification of framework and CDR are based on previous work [26]. Differences in the framework and CDR separated the clones into six groups (I-VI). The CDR3 varies in length and sequence thus most clones are unique, demonstrating diversity is present at birth in the nurse shark.