Figure 1

The EphA2 receptor tyrosine kinase is expressed by in vitro generated LLDC Adherent mononuclear cells were isolated and cultured one week in the presence of GM-CSF and IL-4 (for monocyte derived dendritic cells, DC) and TGF-β (for monocyte derived LLDC). (A) Cells were incubated with a monoclonal EphA2 antibody (bold line) or an irrelevant antibody (dotted line) followed by staining with PE-labeled α-mouse antibody. (B) Cells were incubated with a soluble EphA2 ligand fusion protein (ephrin-A4-Fc, bold line) and a negative control fusion protein (CD19-Fc, dotted line). (C) A Western blot with cell lysates from monocyte derived dendritic cells (DC) or LLDC (LLDC) were immunoblotted using a polyclonal anti-EphA2 antiserum.