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Figure 3 | BMC Immunology

Figure 3

From: Expression and functional effects of Eph receptor tyrosine kinase A family members on Langerhans like dendritic cells

Figure 3

The ephrin-A4 ligand induces signaling through EphA receptors A. Serum starved LLDC were left untreated (untreated) incubated with immobilized ephrin-A4-Fc or a control protein (CD19-Fc) for 10 minutes. The EphA2 receptor was immunoprecipitated, and the phosphorylation status of the EphA2 kinase was analyzed by Western blot analysis with an anti-phosphotyrosine antibody (upper panel). The blot was stripped and reprobed with a polyclonal anti-EphA2 antibody (lower panel). B. Serum starved LLDC were incubated with immobilized ephrin-A1-Fc for 10, 20, and 30 min, and cell lysates were fractioned in cytosol and membrane fraction. Shown in the figure are gel-separated proteins from the membrane fraction hybridized with anti-phosphotyrosine antibody (PY99; upper panel). Arrows indicate phosphorylation or dephosphorylation of distinct proteins during the time course. The filter was stripped and hybridized with an anti-HLA antibody to verify protein loading (lower panel).

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