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Figure 2 | BMC Immunology

Figure 2

From: Fully automated synthesis of (phospho)peptide arrays in microtiter plate wells provides efficient access to protein tyrosine kinase characterization

Figure 2

Reproducibility and specificity of multiplexed EGFR peptide synthesis and ELISA testing Peptides were synthesized as microtiter plate array strips using either tyrosine or phosphotyrosine. Three replicate strips were used, each with no peptide in row A and alternating phosphotyrosine and tyrosine peptides in rows B to H. The strips were tested for reactivity with clone 9H2 antibody as described in Methods. ELISA results (x-axis) are plotted against tyrosine or phosphotyrosine replicates for each strip (y-axis). Standard deviations varied between 5% and 10% of the means. Data are displayed without any correction.

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BMC Immunology

ISSN: 1471-2172

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