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Figure 3 | BMC Immunology

Figure 3

From: Fully automated synthesis of (phospho)peptide arrays in microtiter plate wells provides efficient access to protein tyrosine kinase characterization

Figure 3

Mass spectrometric characterization of EGFR phosphopeptides produced in microtiter plate arrays Panel A illustrates the mass spectrum of the NAEYLRV derivative ([M+H]1+ ion at m/z 1037.56) and panel B for the NAEpYLRV derivative ([M+H]1+ ion at m/z 1170.62). Peptides were released by incubation for 3 hours in TFA-H2O-triisopropylsilane, concentrated in vacuo and reconstituted for MALDI-TOF-TOF analysis. Under these conditions the bis(dimethylamino) phosphate protecting groups of phosphotyrosine were preserved and mass spectra were improved.

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BMC Immunology

ISSN: 1471-2172

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