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Figure 4 | BMC Immunology

Figure 4

From: Fully automated synthesis of (phospho)peptide arrays in microtiter plate wells provides efficient access to protein tyrosine kinase characterization

Figure 4

Distribution of 9H2 phosphotyrosine epitopes in EGFR Overlapping peptide arrays were made and acetylated in microtiter plates using human EGF receptor residues 622–1186 (Swissprot database locus P0053 with leader sequence removed). Each array contained exclusively tyrosine or phosphotyrosine peptides and was composed of 92 peptides each of which was 21 amino acids long and offset from its neighbor by 6 amino acids. Array peptide wells were reacted in multiplexed ELISA format with antibody 9H2 as described in Methods. Data are plotted without any corrections. Peptide sequences and raw data are listed [see Additional file 1].

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BMC Immunology

ISSN: 1471-2172

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