Expression of human AID in yeast induces mutations in context similar to the context of somatic hypermutation at G-C pairs in immunoglobulin genes

Table 5 Mutations in different mutable motifs in different spectra.

Spectrum	Sequence motif (mutable positions are underlined)		Reference
	DG YW / WRC H	NG YW / WRC N
Spontaneous can1 mutations in the wild-type yeast	0.5 / 0.5	0.7/0.5	[52]
Spontaneous SUP4-o mutations in ung1 yeast strain	0.9/1.1	0.9/1.1	[60]
hAIDSc in the CAN1 in wild-type yeast	*1.2 / 5.2*	*1.5 / 4.8*	This work
hAIDSc in the CAN1 in ung1 yeast	*2.0 / 1.8*	*3.2 / 1.6*	This work
SHM in V_κOx1 in mouse	*3.5 / 2.5*	*3.4 / 2.5*	[47]
SHM in J_H4 region in wild-type mouse	*4.4 / 3.2*	*4.4 / 3.2*	[26]
SHM in J_H4 region in Ung1^-/- mouse	*4.5 / 3.6*	*4.5 / 3.6*	[26]
SHM in J_H4 region in Ung1^-/- Msh2^-/- mouse	*3.3 / 5.0*	*3.2 / 5.0*	[67]
AID in GFP in human fibroblasts	*7.6 / 4.8*	*7.1 / 4.1*	[13]
AID in sacB in E.coli	*2.3 / 3.7*	*1.6 / 4.6*	[36]
AID in lacZ in vitro	*-- / 2.1*	*-- / 2.2*	[40]

The values listed represent the fold increase in occurrence of mutations at mutable motifs above the average occurrence of mutations at other G-C sites. Number of mutations in mutable motifs was calculated for the underlined bases. Bold italicized values represent a statistically significant correlation (P ≤ 0.05) between a mutable motif and the distribution of mutations, as revealed by using a Monte Carlo procedure [7].

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