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Table 6 Base substitution hotspots and mutable motifs.

From: Expression of human AID in yeast induces mutations in context similar to the context of somatic hypermutation at G-C pairs in immunoglobulin genes

Number of substitutions

Position

Sequence

Wild-type

ung1

NG YW/WRC N variant (N = A/T/C/G)

238

GTA C AGA

4

2

TG YW

268

AAG C AAA

7

2

TG YW

299

GTG G TA C

1

4

GG YW

424

GGT G AAA

0

3

-

612

ATG G AAT

0

3

-

896

AAG G TA C

3

1

GG YW

980

TCC G TA T

2

6

CG YW

1166

CTG C CGC

4

0

GG YW

1392

ATG G TTA

3

2

GG YW

1426

ATG C AAG

1

4

TG YW

1486

ATG C CCG

3

0

GG YW

  1. The CLUSTERM program [97] was used for hotspot analysis. The hotspot threshold value is three mutations for both spectra. Hotspot motifs are shown in Bold and enlarged font. The hotspot base is underlined.