Figure 7
P53 associates with ΔA5-IκBα in UV-irradiated melanoma cells. A2085 cells stably transfected with the parent vector (Con) or ΔA5-IκBα allele (DA5) were irradiated for 30" with UVB and harvested 4 hrs later. The extracts were subjected to immunoprecipitation with non specific rabbit serum (ns), polyclonal rabbit anti-HA antibody (α-HA), or mouse monoclonal anti-p53 Ab2 antibody (α-p53) and probed by western analysis with mouse anti-IκBα antibody directed at the C-terminus of IκBα (top panel) or the Ab3 anti-p53 monoclonal antibody (bottom panel). Experiments were performed on stably transfected, pooled transfectants of controls (Con) or ΔA5-IκBα (DA5).