Figure 9

Comparison of liquid and lyophilised reagents. Comparative results are shown with backgrounds subtracted; no significant differences in backgrounds were seen with liquid versus lyophilised reagents (data not shown). (A) Data from one site that compared cocktail 1 (CD4 FITC/IFNγ +IL-2 PE/CD8 PerCP-Cy5.5/CD3 APC) in liquid and lyophilised form in Experiment 4. Black bars indicate liquid antibodies, grey bars indicate lyophilised antibodies. Error bars indicate SEM of duplicate wells. (B) Combined comparison of liquid antigen + liquid antibodies versus lyophilised antigen + lyophilised antibodies. Whole blood was activated with either SEB or pp65 peptide mix, and the percentage of IFNγ⁺ cells (CD4⁺ or CD8⁺) were compared with liquid versus lyophilised reagents (left panel). A similar comparison was made for the mean fluorescence intensity (MFI) of IFNγ⁺ cells (CD4⁺ or CD8⁺) (right panel). Similar results were obtained using PBMC (not shown).