Figure 2
Time-dependent secretion of Mycobacterium -, and LPS- induced CCL2, CXCL8 and TNFα. Whole blood cells (106) from healthy controls (N = 12) were stimulated with M. bovis BCG (2 × 105 CFU/ml, ratio of 0.2 bacilli per cell) and LPS at 0.1 μg/ml. Supernatants were harvested at 1, 6, 18, 48 and 120 h post-stimulation and tested for cytokine activity. Mean values are illustrated with SD indicated as 'y' error bars. A. CCL2, B. CXCL8, C. TNFα. Un-stimulated cells (spon) '◇', BCG stimulated '■' and LPS stimulated '▲'.