Chemotaxis assay in U-937/rabbit CCR2 stable transfectants. Chemotaxis assays were performed in parental U-937 or U-937/rabbit CCR2 stable transfectants using increasing concentrations of human MCP-1 (A), mouse JE (B) or human SDF-1α (C). The effect of TAK-779 on rabbit CCR2-induced chemotaxis was tested in the presence of 4 nM of mouse JE (D) or hMCP-1 (E). Effect of TAK-779 on hCCR2-induced chemotaxis in the presence of 4 nM of hMCP-1 (F). Each point represents means of the triplicate from one assay.