Figure 1
From: Estren promotes androgen phenotypes in primary lymphoid organs and submandibular glands
Estren reduces thymus weight independently of estrogen receptors. Ovariectomized 3-month-old female C57/B16 mice were treated for 18–21 days with daily s.c. injections of the estrogen receptor antagonist ICI 182,780 (200 μg/day) or vehicle Miglyol 812. Simultaneously, both the vehicle and the ICI groups were given daily s.c injections of either E2 (0.7 μg/day), estren (75 μg/day) or control Miglyol 812 oil. ICI 182,780 blocked the inhibitory effect of E2 on thymus weight. Treatment with estren resulted in lower thymus weight in both vehicle and ICI mice. One-way ANOVA followed by Fisher's test was used to compare data from mice in different treatment groups. Results are presented as mean ± standard deviation. * P < 0.05, *** P < 0.001.